recombinant mouse cxcl12 460 sd Search Results


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R&D Systems recombinant mouse cxcl12 460 sd
Recombinant Mouse Cxcl12 460 Sd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant mouse cxcl12 sdf 10l
Recombinant Mouse Cxcl12 Sdf 10l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems cxcl12
Cxcl12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant mouse cxcl12 sdf 1α protein
NAR inhibited the polarization of primary CD4 + T lymphocytes. ( A ) F-actin of CD4 + T lymphocytes measured by immunofluorescence. ( B ) Representative western blots showing the expression of pMLC in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. <t>CXCL12</t> group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).
Recombinant Mouse Cxcl12 Sdf 1α Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse cxcl12 sdf 1α protein/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant mouse cxcl12 sdf 1α protein - by Bioz Stars, 2026-03
93/100 stars
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Image Search Results


NAR inhibited the polarization of primary CD4 + T lymphocytes. ( A ) F-actin of CD4 + T lymphocytes measured by immunofluorescence. ( B ) Representative western blots showing the expression of pMLC in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).

Journal: International Journal of Molecular Sciences

Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission

doi: 10.3390/ijms24010279

Figure Lengend Snippet: NAR inhibited the polarization of primary CD4 + T lymphocytes. ( A ) F-actin of CD4 + T lymphocytes measured by immunofluorescence. ( B ) Representative western blots showing the expression of pMLC in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).

Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China), recombinant mouse CXCL12/SDF-1α protein (Cat. No. 460-SD-050; R&D Systems, Minneapolis, MN, USA), ultra-LEAF™ purified anti-mouse CD3 antibody (Cat. No. 100238; Biolegend, San Diego, CA, USA), ultra-LEAF™ purified anti-mouse CD28 antibody (Cat. No. 102116; Biolegend), rat-IL-6-HS ELISA kit (Cat. No. EK306HS-96; MultiSciences, Hangzhou, China), rat-TNF-α-HS (Cat. No. EK382HS-96; MultiSciences), mitochondrial membrane potential assay kit with JC-1 (Cat. No. C2006, Beyotime), mitoSOX red mitochondrial superoxide indicator (Cat. No. LX3608; Warbio, Nanjing, China), coraLite ® 594-Phalloidine (red) (Cat. No. PF00003; proteintech, Singapore), MACS buffer (Cat. No. 130-091-221, Miltenyi, Bergisch Gladbach, Germany), CD4 + T Cell Isolation Kit (mouse) (Cat. No. 130-104-454, Miltenyi), Transwell (5.0 μm) (Cat. No. 3421, Corning, Corning, NY, USA), poly-lysine coated slides (Cat. No. 188158; LIUSHENG, Nantong, China).

Techniques: Immunofluorescence, Western Blot, Expressing, Control

NAR inhibited the mitochondrial distribution and migration of primary CD4 + T lymphocytes. ( A ) Mitochondria (TOM20) and the uropod marker ICAM1 in different groups. The percentage of cells with co-localized mitochondria and ICAM1 is reported in the graph (n = 3 mice per group). Control group ( B ), CXCL12 group ( C ), CXCL12 + Mdivi-1 ( D ), CXCL12 + NAR ( E ) CD4 + T lymphocytes were stained for the indicated proteins (F-actin (detected using fluorescent phalloidin); ICAM-1; and TOM20) and (left) imaged by confocal microscopy; the uropod is marked with ICAM-1. (Right) Fluorescence intensity analysis was performed from the uropod to the leading edge (L.E.) over the line represented at the merge image. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).

Journal: International Journal of Molecular Sciences

Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission

doi: 10.3390/ijms24010279

Figure Lengend Snippet: NAR inhibited the mitochondrial distribution and migration of primary CD4 + T lymphocytes. ( A ) Mitochondria (TOM20) and the uropod marker ICAM1 in different groups. The percentage of cells with co-localized mitochondria and ICAM1 is reported in the graph (n = 3 mice per group). Control group ( B ), CXCL12 group ( C ), CXCL12 + Mdivi-1 ( D ), CXCL12 + NAR ( E ) CD4 + T lymphocytes were stained for the indicated proteins (F-actin (detected using fluorescent phalloidin); ICAM-1; and TOM20) and (left) imaged by confocal microscopy; the uropod is marked with ICAM-1. (Right) Fluorescence intensity analysis was performed from the uropod to the leading edge (L.E.) over the line represented at the merge image. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).

Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China), recombinant mouse CXCL12/SDF-1α protein (Cat. No. 460-SD-050; R&D Systems, Minneapolis, MN, USA), ultra-LEAF™ purified anti-mouse CD3 antibody (Cat. No. 100238; Biolegend, San Diego, CA, USA), ultra-LEAF™ purified anti-mouse CD28 antibody (Cat. No. 102116; Biolegend), rat-IL-6-HS ELISA kit (Cat. No. EK306HS-96; MultiSciences, Hangzhou, China), rat-TNF-α-HS (Cat. No. EK382HS-96; MultiSciences), mitochondrial membrane potential assay kit with JC-1 (Cat. No. C2006, Beyotime), mitoSOX red mitochondrial superoxide indicator (Cat. No. LX3608; Warbio, Nanjing, China), coraLite ® 594-Phalloidine (red) (Cat. No. PF00003; proteintech, Singapore), MACS buffer (Cat. No. 130-091-221, Miltenyi, Bergisch Gladbach, Germany), CD4 + T Cell Isolation Kit (mouse) (Cat. No. 130-104-454, Miltenyi), Transwell (5.0 μm) (Cat. No. 3421, Corning, Corning, NY, USA), poly-lysine coated slides (Cat. No. 188158; LIUSHENG, Nantong, China).

Techniques: Migration, Marker, Control, Staining, Confocal Microscopy, Fluorescence

NAR inhibited the mitochondrial fission of primary CD4 + T lymphocytes. ( A ) Drp1 and Fis1 as measured by immunofluorescence. Representative Western blots showing the expression of Drp1 ( B ) and Fis1 ( C ) in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3–4 mice per group, 3 sections and 3–5 different fields of each section were randomly selected for semi-quantitative evaluation).

Journal: International Journal of Molecular Sciences

Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission

doi: 10.3390/ijms24010279

Figure Lengend Snippet: NAR inhibited the mitochondrial fission of primary CD4 + T lymphocytes. ( A ) Drp1 and Fis1 as measured by immunofluorescence. Representative Western blots showing the expression of Drp1 ( B ) and Fis1 ( C ) in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3–4 mice per group, 3 sections and 3–5 different fields of each section were randomly selected for semi-quantitative evaluation).

Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China), recombinant mouse CXCL12/SDF-1α protein (Cat. No. 460-SD-050; R&D Systems, Minneapolis, MN, USA), ultra-LEAF™ purified anti-mouse CD3 antibody (Cat. No. 100238; Biolegend, San Diego, CA, USA), ultra-LEAF™ purified anti-mouse CD28 antibody (Cat. No. 102116; Biolegend), rat-IL-6-HS ELISA kit (Cat. No. EK306HS-96; MultiSciences, Hangzhou, China), rat-TNF-α-HS (Cat. No. EK382HS-96; MultiSciences), mitochondrial membrane potential assay kit with JC-1 (Cat. No. C2006, Beyotime), mitoSOX red mitochondrial superoxide indicator (Cat. No. LX3608; Warbio, Nanjing, China), coraLite ® 594-Phalloidine (red) (Cat. No. PF00003; proteintech, Singapore), MACS buffer (Cat. No. 130-091-221, Miltenyi, Bergisch Gladbach, Germany), CD4 + T Cell Isolation Kit (mouse) (Cat. No. 130-104-454, Miltenyi), Transwell (5.0 μm) (Cat. No. 3421, Corning, Corning, NY, USA), poly-lysine coated slides (Cat. No. 188158; LIUSHENG, Nantong, China).

Techniques: Immunofluorescence, Western Blot, Expressing, Control

NAR reduced the co-localization of Drp1 with TOM20 as well as Drp1 with Fis1 in primary CD4 + T lymphocytes. ( A ) Co-localization of Drp1 with TOM20. ( B ) Co-localization of Drp1 with Fis1. * p < 0.05 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ±SEM (n = 3 mice per group).

Journal: International Journal of Molecular Sciences

Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission

doi: 10.3390/ijms24010279

Figure Lengend Snippet: NAR reduced the co-localization of Drp1 with TOM20 as well as Drp1 with Fis1 in primary CD4 + T lymphocytes. ( A ) Co-localization of Drp1 with TOM20. ( B ) Co-localization of Drp1 with Fis1. * p < 0.05 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ±SEM (n = 3 mice per group).

Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China), recombinant mouse CXCL12/SDF-1α protein (Cat. No. 460-SD-050; R&D Systems, Minneapolis, MN, USA), ultra-LEAF™ purified anti-mouse CD3 antibody (Cat. No. 100238; Biolegend, San Diego, CA, USA), ultra-LEAF™ purified anti-mouse CD28 antibody (Cat. No. 102116; Biolegend), rat-IL-6-HS ELISA kit (Cat. No. EK306HS-96; MultiSciences, Hangzhou, China), rat-TNF-α-HS (Cat. No. EK382HS-96; MultiSciences), mitochondrial membrane potential assay kit with JC-1 (Cat. No. C2006, Beyotime), mitoSOX red mitochondrial superoxide indicator (Cat. No. LX3608; Warbio, Nanjing, China), coraLite ® 594-Phalloidine (red) (Cat. No. PF00003; proteintech, Singapore), MACS buffer (Cat. No. 130-091-221, Miltenyi, Bergisch Gladbach, Germany), CD4 + T Cell Isolation Kit (mouse) (Cat. No. 130-104-454, Miltenyi), Transwell (5.0 μm) (Cat. No. 3421, Corning, Corning, NY, USA), poly-lysine coated slides (Cat. No. 188158; LIUSHENG, Nantong, China).

Techniques: Control