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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission
doi: 10.3390/ijms24010279
Figure Lengend Snippet: NAR inhibited the polarization of primary CD4 + T lymphocytes. ( A ) F-actin of CD4 + T lymphocytes measured by immunofluorescence. ( B ) Representative western blots showing the expression of pMLC in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).
Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China),
Techniques: Immunofluorescence, Western Blot, Expressing, Control
Journal: International Journal of Molecular Sciences
Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission
doi: 10.3390/ijms24010279
Figure Lengend Snippet: NAR inhibited the mitochondrial distribution and migration of primary CD4 + T lymphocytes. ( A ) Mitochondria (TOM20) and the uropod marker ICAM1 in different groups. The percentage of cells with co-localized mitochondria and ICAM1 is reported in the graph (n = 3 mice per group). Control group ( B ), CXCL12 group ( C ), CXCL12 + Mdivi-1 ( D ), CXCL12 + NAR ( E ) CD4 + T lymphocytes were stained for the indicated proteins (F-actin (detected using fluorescent phalloidin); ICAM-1; and TOM20) and (left) imaged by confocal microscopy; the uropod is marked with ICAM-1. (Right) Fluorescence intensity analysis was performed from the uropod to the leading edge (L.E.) over the line represented at the merge image. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3 mice per group).
Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China),
Techniques: Migration, Marker, Control, Staining, Confocal Microscopy, Fluorescence
Journal: International Journal of Molecular Sciences
Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission
doi: 10.3390/ijms24010279
Figure Lengend Snippet: NAR inhibited the mitochondrial fission of primary CD4 + T lymphocytes. ( A ) Drp1 and Fis1 as measured by immunofluorescence. Representative Western blots showing the expression of Drp1 ( B ) and Fis1 ( C ) in the CD4 + T lymphocyte in different groups. ** p < 0.01 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ± SEM (n = 3–4 mice per group, 3 sections and 3–5 different fields of each section were randomly selected for semi-quantitative evaluation).
Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China),
Techniques: Immunofluorescence, Western Blot, Expressing, Control
Journal: International Journal of Molecular Sciences
Article Title: The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4 + T Lymphocyte Driven by Regulating Mitochondrial Fission
doi: 10.3390/ijms24010279
Figure Lengend Snippet: NAR reduced the co-localization of Drp1 with TOM20 as well as Drp1 with Fis1 in primary CD4 + T lymphocytes. ( A ) Co-localization of Drp1 with TOM20. ( B ) Co-localization of Drp1 with Fis1. * p < 0.05 vs. control group, # p < 0.05 vs. CXCL12 group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 5 µm. The data are presented as means ±SEM (n = 3 mice per group).
Article Snippet: Immunization Grade Bovine Type II Collagen (Cat. No. 20021; Chondrex, Woodinville, WA, USA), NAR (Cat. No. B21596; Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), mitochondrial division inhibitor 1 (Mdivi-1) (Cat. No. SC8028; Beyotime, Nantong, China),
Techniques: Control